![]() Track the number of incoming successes from the control group. Track the number of incoming successes from the treatment group. Assign subjects randomly to the treatment and control, with 50 probability each. This open-source software can serve as a useful, high-throughput tool for analyzing cardiomyocyte physiology from fluorescence imaging. The sequential procedure works like this: At the beginning of the experiment, choose a sample size N. However, we also observed marked calcium transient suppression, highlighting the advantage of testing multiple physiologic readouts in cardiomyocytes rather than relying on heterologous overexpression of single channels such as the human ether-a-go-go-related gene channel. Consistent with clinical observations, our system predicted mild action potential prolongation. We then demonstrate the software by assessing changes to action potentials and calcium transients in our micro-heart muscles after exposure to hydroxychloroquine, an antimalarial drug with known cardiac liability. We first identified the most robust manner in which to automatically identify the initiation point for action potentials and calcium transients in a user-independent manner, and used this approach to quantify the duration and morphology of these signals. ![]() In this study, we describe an automated, open-source MATLAB-based analysis software for calculating cardiac action potentials and calcium transients from fluorescent reporters. Induced pluripotent stem cell-derived cardiomyocytes and tissues fabricated from these cells are promising as screening tools for early identification of these potential cardiac liabilities. Drugs are often removed from clinical trials or market progression owing to their unforeseen effects on cardiac action potential and calcium handling.
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